Dipole Emitter Lateral Point Spread Function Communicates Orientation and Axial Position

نویسندگان

چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Position and orientation estimation of fixed dipole emitters using an effective Hermite point spread function model.

We introduce a method for determining the position and orientation of fixed dipole emitters based on a combination of polarimetry and spot shape detection. A key element is an effective Point Spread Function model based on Hermite functions. The model offers a good description of the shape variations with dipole orientation and polarization detection channel, and provides computational advantag...

متن کامل

Estimation Methods of the Point Spread Function Axial Position: A Comparative Computational Study

The precise knowledge of the point spread function is central for any imaging system characterization. In fluorescence microscopy, point spread function (PSF) determination has become a common and obligatory task for each new experimental device, mainly due to its strong dependence on acquisition conditions. During the last decade, algorithms have been developed for the precise calculation of t...

متن کامل

Improved axial resolution by point-spread autocorrelation function imaging.

Based on an interferometric spatial autocorrelation of two shifted point-spread functions combined with confocal detection, we demonstrate that the point-spread autocorrelation function technique provides improved axial resolution compared with conventional confocal imaging. The principle of the technique is demonstrated for a one-sided f luorescing step object.

متن کامل

Evaluation of the Effect of Tumor Position on Standardized Uptake Value Using Time-of-Flight Reconstruction and Point Spread Function

Objective(s): The present study was conducted to examine whether the standardized uptake value (SUV) may be affected by the spatial position of a lesion in the radial direction on positron emission tomography (PET) images, obtained via two methods based on time-of-flight (TOF) reconstruction and point spread function (PSF). Methods: A cylinder phantom with the sphere (30mm diameter), located in...

متن کامل

Single Molecule Fluorescence Image Patterns Linked to Dipole Orientation and Axial Position: Application to Myosin Cross-Bridges in Muscle Fibers

BACKGROUND Photoactivatable fluorescent probes developed specifically for single molecule detection extend advantages of single molecule imaging to high probe density regions of cells and tissues. They perform in the native biomolecule environment and have been used to detect both probe position and orientation. METHODS AND FINDINGS Fluorescence emission from a single photoactivated probe cap...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Biophysical Journal

سال: 2011

ISSN: 0006-3495

DOI: 10.1016/j.bpj.2010.12.1939